A small volume of test substance is added directly to the center of the carrier (on top of the dried inoculum) and allowed to sit undisturbed for the duration of the exposure period.After drying, the carriers are individually placed into the bottom of sterile vessels for testing.The test microorganism is dried under vacuum at room temperature.A small volume of test microorganism is added to the center of 1 cm diameter brushed stainless steel disks.
Microchem Laboratory stays abreast of method changes and ensures that clients' studies benefit from the most current methodology. EPA, particularly with regard to endospore preparation and storage requriements, as well as soil load. The method is being actively developed and refined by the U.S. EPA to substantiate efficacy claims for disinfectants against endospores of Clostridium difficile. This method has been accepted by the U.S. The ASTM E2197 method is designed to test bactericidal, fungicidal, sporicidal, and virucidal capabilities of liquid germicides on non-porous hard surfaces using a 1 cm diameter brushed stainless steel surface.
The Science of Microbial Identification Using DNA Sequencing.Introduction to High Level Disinfectant Testing.